Prioritizing Proteomics Assay Development for Clinical Translation

Expediting SRM Assay Development for Large-Scale Targeted Proteomics Experiments

Because of its high sensitivity and specificity, selected reaction monitoring (SRM)-based targeted proteomics has become increasingly popular for biological and translational applications. Selection of optimal transitions and optimization of collision energy (CE) are important assay development steps for achieving sensitive detection and accurate quantification; however, these steps can be labo...

DEVELOPMENT OF A SIMPLE AND SENSITIVE ENZYME- LINKED IMMUNOSORBENT ASSAY (ELISA) FOR CLINICAL MEASUREMENT OF TESTOSTERONE USING PENICILLINASE AS LABEL

An enzyme-linked immunosorbent assay using a homologous combination of antiserum raised against testosterone-3-0-carboxymethyloxime-bovine serum albumin (T-3-0-CMO-BSA ) and penicillinase-labelled T-3-0-CMO was developed. This assay was utilized to measure testosterone in serum samples of male and female subjects. The sensitivity of the assay is 50pg/well and the antibody developed crossrea...

Application of Proteomics Technologies in the Drug Development Process

Clinical proteomics.

Clinical Proteomics is an emerging interdisciplinary research field that coalesces researchers from many different areas of biomedical research into one of the most likely disciplines to successfully foster the translation of basic scientific knowledge into clinical applications for the benefit of the patient. Major research interests that are expected to profit from the application of proteomi...

Application of Proteomics Technologies in the Drug Development Process